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Welcome to the knowledge base of Ucan-U. Ucan-U wants to use this wiki to share relevant information related to its work. Currently Ucan-U only shares standard operating procedures in this system, but with your help we hope to be able to collect and organise a wealth of information in here.

 
<h2>Standard operating procedures</h2>
 
<h3>Flow cytometry</h3>
<ol>
<li><a title="Immunofluorescent staining and flow cytometric analysis of cells" href="http://www.ucan-u.org/kb/immunofluorescent-staining-and-flow-cytometric-analysis-of-cells/">Immunofluorescent staining and flow cytometric analysis of cells</a></li>
<li><a title="Immunofluorescent staining and flow cytometric analysis of human CD4+ Regulatory T cells" href="http://www.ucan-u.org/kb/immunofluorescent-staining-and-flow-cytometric-analysis-of-human-cd4-regulatory-t-cells/">Immunofluorescent staining and flow cytometric analysis of human CD4+ Regulatory T cells</a></li>
<li><a title="General Annexin V Staining Procedure" href="http://www.ucan-u.org/kb/general-annexin-v-staining-procedure/">General Annexin V Staining Procedure</a></li>
<li><a title="Proliferation of Human Mononuclear Cells with CFSE" href="http://www.ucan-u.org/kb/proliferation-of-human-mononuclear-cells-with-cfse/">Proliferation of Human Mononuclear Cells with CFSE</a></li>
</ol>
 
<h3>Other</h3>
<ol>
<li><a title="Detection of proteins of interest in cell lysates" href="http://www.ucan-u.org/kb/detection-of-proteins-of-interest-in-cell-lysates/">Detection of proteins of interest in cell lysates</a></li>
<li><a title="Generation of EBV-immortalized B cell lines" href="http://www.ucan-u.org/kb/generation-of-ebv-immortalized-b-cell-lines/">Generation of EBV-immortalized B cell lines</a></li>
<li><a title="Isolation of Human Mononuclear Cells from Blood" href="http://www.ucan-u.org/kb/isolation-of-human-mononuclear-cells-from-blood/">Isolation of Human Mononuclear Cells from Blood</a></li>
<li><a title="Isolation of Human Mononuclear Cells from Synovial Fluid" href="http://www.ucan-u.org/kb/isolation-of-human-mononuclear-cells-from-synovial-fluid/">Isolation of Human Mononuclear Cells from Synovial Fluid</a></li>
<li><a title="Lysis of Whole blood and Human Mononuclear Cells from Blood and Synovial Fluid" href="http://www.ucan-u.org/kb/lysis-of-whole-blood-and-human-mononuclear-cells-from-blood-and-synovial-fluid/">Lysis of Whole blood and Human Mononuclear Cells from Blood and Synovial Fluid</a></li>
<li><a title="Plasma Isolation from Human Peripheral Blood" href="http://www.ucan-u.org/kb/plasma-isolation-from-human-peripheral-blood/">Plasma Isolation from Human Peripheral Blood</a></li>
<li>Proliferation of Human Mononuclear Cells with 3H-Thymidine</li>
<li>Serum Isolation from Human Peripheral Blood</li>
<li>Thawing Human Mononuclear Cells</li>
</ol>